A COLORIMETRIC DETERMINATION OF OROTIC ACID

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Determination of Orotic acid in milk

Method VFD0134J_E HPLC Column: Eurokat H, 10 μm, 300 x 8 mm Order No. 30GX340EKN Phase: Eurokat H, 10 μm Eluent: 0.01 N Sulfuric acid Gradient: Isocratic Flow rate: 0.6 ml/min Temperature: 60 °C Volume: 20 μL (Standard) Detection: UV, 210 nm Substances: Orotic acid, Lactic acid and Acetic acid

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Determination of orotic acid in whey and modified whey products.

The concentration of orotic acid in dry whey ranged between 64 and 146 mg/100 g and appeared dependent on whey type. The orotic acid of modified whey products, prepared commercially by electrodialysis, ultrafiltration, gel filtration, or polyphosphate precipitation was between 7 and 124 mg/100 g, which was less than that of dry whey. It appears that whey processing methods do not concentrate or...

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Orotic acid in milk.

Other studies in this laboratory (1) drew our attention to the possibility that erotic acid might be concerned with the maintenance of adequate lactation in the rat and with the survival of the young. Since no data were available on the erotic acid content of milk, this study was undertaken. The occurrence of erotic acid as a component of milk has been indicated since 1904, when Biscaro and Bel...

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Orotic Acid Feeding

1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5a-[4-14C]androstane-3a,17f8-diol, [4-4C]cholesterol, 7a-hydroxy4-6fi-3H]cholesten3-one, 5fi-[7fi-3H]cholestane-3a,7U-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty li...

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Redetermination of orotic acid monohydrate

The crystal structure of the title compound, which is also known as vitamin B(13) (systematic name: 2,6-dioxo-1,2,3,6-tetra-hydro-pyrimidine-4-carboxylic acid monohydrate), C(5)H(4)N(2)O(4)·H(2)O, was reported for the first time by Takusagawa & Shimada [Bull. Chem. Soc. Jpn (1973 ▶), 46, 2011-2019]. The present redetermination provides more precise values of the mol-ecular geometry. The asymmet...

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ژورنال

عنوان ژورنال: THE JOURNAL OF VITAMINOLOGY

سال: 1963

ISSN: 0022-5398

DOI: 10.5925/jnsv1954.9.217